...rare book collections at the University of Rochester, the Rochester Institute of Technology (RIT), Eastman House, and the Strong Museum; plus two literary landmarks; and six (!) used bookstores. (Also, Yesterday's Muse in Webster, NY, is just east of town.)
Here in Syracuse, NY where I live we don't rate so highly but there is an ongoing free exhibition at the Rare Book Archives of Syracuse University (top floor of the Bird Library) entitled The Power and the Piety highlighting selections of books form their collection around the threshold of the invention of printing. They have beautiful illuminated manuscripts, an early copy of Machiavelli's The Prince, a page from a Gutenberg Bible and many other pieces up. The exhibition lasts until June 22nd.